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polyclonal rabbit anti phosphopkc pan antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc polyclonal rabbit anti phosphopkc pan antibodies
    Polyclonal Rabbit Anti Phosphopkc Pan Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+phosphopkc/NOS+(pan)+Antibody/pm18290605-36-8-16
    Average 94 stars, based on 90 article reviews
    polyclonal rabbit anti phosphopkc pan antibodies - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Phospho-proteomics:

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.
    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).. Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.

    Control:

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.
    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).. Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.

    Isolation:

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.
    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).. Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.

    SDS Page:

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.
    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).. Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.

    Membrane:

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.
    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).. Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.

    Western Blot:

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.
    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).. Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.Rabbit polyclonal anti-active MAPK (1/5000) was purchased from Promega, and anti-total PKC (1.5 mg/ml) and anti-total PKC (1.5 g/ml) were from Upstate Biotechnology.



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    polyclonal rabbit anti phosphopkc pan antibodies  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc polyclonal rabbit anti phosphopkc pan antibodies
    Polyclonal Rabbit Anti Phosphopkc Pan Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+phosphopkc/NOS+(pan)+Antibody/pm18290605-36-8-16
    Average 94 stars, based on 1 article reviews
    polyclonal rabbit anti phosphopkc pan antibodies - by Bioz Stars, 2026-09
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    rabbit polyclonal anti phosphopkc  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit polyclonal anti phosphopkc
    FIGURE 1. Decreased PMA-Raf-1-Erk pathway phosphorylation in lupus T cells. A, CD4 T cells from a healthy control (N) or from two different patients with SLE (L1, L2) were isolated and immediately stimulated with 50 ng/ml PMA for 15 min or not stimulated as described in Materials and Methods. Proteins from whole cell lysates were fractionated by SDS-PAGE, transferred to nitrocellulose membranes and probed with a <t>polyclonal</t> Ab against the active dually phosphorylated form of ERK1/2. The membrane was stripped and reblotted sequentially for phospho-MEK1/2 Ser217/221 and Raf-1 p-Ser338. -actin was used as loading control. B, Quantitative immunoblot analysis of phospho-ERK, phospho-MEK, and phospho-Raf in CD4 T cells from three to six different patients with active lupus treated as in A and compared with normal donors. Values were normalized to -actin. The relative phosphorylation of the different kinases in PMA-stimulated CD4 lupus T cells was compared with normal-treated T cells arbitrarily considered as 100%. Results shown are the mean percentage of phosphorylation SD of the indicated number of independent experiments.
    Rabbit Polyclonal Anti Phosphopkc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+phosphopkc/Phospho-PKCalpha%2Fbeta+II+(Thr638%2F641)+Antibody/pm17911642-69-6-25
    Average 95 stars, based on 1 article reviews
    rabbit polyclonal anti phosphopkc - by Bioz Stars, 2026-09
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    polyclonal antibody against phosphopkc pan  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc polyclonal antibody against phosphopkc pan
    FIGURE 1. Decreased PMA-Raf-1-Erk pathway phosphorylation in lupus T cells. A, CD4 T cells from a healthy control (N) or from two different patients with SLE (L1, L2) were isolated and immediately stimulated with 50 ng/ml PMA for 15 min or not stimulated as described in Materials and Methods. Proteins from whole cell lysates were fractionated by SDS-PAGE, transferred to nitrocellulose membranes and probed with a <t>polyclonal</t> Ab against the active dually phosphorylated form of ERK1/2. The membrane was stripped and reblotted sequentially for phospho-MEK1/2 Ser217/221 and Raf-1 p-Ser338. -actin was used as loading control. B, Quantitative immunoblot analysis of phospho-ERK, phospho-MEK, and phospho-Raf in CD4 T cells from three to six different patients with active lupus treated as in A and compared with normal donors. Values were normalized to -actin. The relative phosphorylation of the different kinases in PMA-stimulated CD4 lupus T cells was compared with normal-treated T cells arbitrarily considered as 100%. Results shown are the mean percentage of phosphorylation SD of the indicated number of independent experiments.
    Polyclonal Antibody Against Phosphopkc Pan, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+phosphopkc/CaMKII+(pan)+Rabbit+mAb/pm16987344-61-1-10
    Average 96 stars, based on 1 article reviews
    polyclonal antibody against phosphopkc pan - by Bioz Stars, 2026-09
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    Image Search Results


    FIGURE 1. Decreased PMA-Raf-1-Erk pathway phosphorylation in lupus T cells. A, CD4 T cells from a healthy control (N) or from two different patients with SLE (L1, L2) were isolated and immediately stimulated with 50 ng/ml PMA for 15 min or not stimulated as described in Materials and Methods. Proteins from whole cell lysates were fractionated by SDS-PAGE, transferred to nitrocellulose membranes and probed with a polyclonal Ab against the active dually phosphorylated form of ERK1/2. The membrane was stripped and reblotted sequentially for phospho-MEK1/2 Ser217/221 and Raf-1 p-Ser338. -actin was used as loading control. B, Quantitative immunoblot analysis of phospho-ERK, phospho-MEK, and phospho-Raf in CD4 T cells from three to six different patients with active lupus treated as in A and compared with normal donors. Values were normalized to -actin. The relative phosphorylation of the different kinases in PMA-stimulated CD4 lupus T cells was compared with normal-treated T cells arbitrarily considered as 100%. Results shown are the mean percentage of phosphorylation SD of the indicated number of independent experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Impaired T cell protein kinase C delta activation decreases ERK pathway signaling in idiopathic and hydralazine-induced lupus.

    doi: 10.4049/jimmunol.179.8.5553

    Figure Lengend Snippet: FIGURE 1. Decreased PMA-Raf-1-Erk pathway phosphorylation in lupus T cells. A, CD4 T cells from a healthy control (N) or from two different patients with SLE (L1, L2) were isolated and immediately stimulated with 50 ng/ml PMA for 15 min or not stimulated as described in Materials and Methods. Proteins from whole cell lysates were fractionated by SDS-PAGE, transferred to nitrocellulose membranes and probed with a polyclonal Ab against the active dually phosphorylated form of ERK1/2. The membrane was stripped and reblotted sequentially for phospho-MEK1/2 Ser217/221 and Raf-1 p-Ser338. -actin was used as loading control. B, Quantitative immunoblot analysis of phospho-ERK, phospho-MEK, and phospho-Raf in CD4 T cells from three to six different patients with active lupus treated as in A and compared with normal donors. Values were normalized to -actin. The relative phosphorylation of the different kinases in PMA-stimulated CD4 lupus T cells was compared with normal-treated T cells arbitrarily considered as 100%. Results shown are the mean percentage of phosphorylation SD of the indicated number of independent experiments.

    Article Snippet: The following primary Abs were used: rabbit polyclonal anti-phosphoPKC (Thr638/641), anti-phospho-PKC (Thr538), anti-phospho-PKC (Thr505), anti-phospho-Raf (Ser338), anti-phospho-MEK1/2 (Ser217/221), and anti MEK1/2 used at 1/1000 dilution (Cell Signaling Technology).

    Techniques: Phospho-proteomics, Control, Isolation, SDS Page, Membrane, Western Blot